Influence of Poly(ethylene glycol) Molecular Architecture on Particle Assembly and <i>Ex Vivo</i> Particle–Immune Cell Interactions in Human Blood

نویسندگان

چکیده

Poly(ethylene glycol) (PEG) is widely used in particle assembly to impart biocompatibility and stealth-like properties vivo for diverse biomedical applications. Previous studies have examined the effect of PEG molecular weight coating density on biological fate various particles; however, there are few that detail fundamental role architecture engineering bio–nano interactions. Herein, we engineered particles using a mesoporous silica (MS) templating method investigated how building block impacted physicochemical (e.g., surface chemistry mechanical characteristics) subsequently modulated particle–immune cell interactions human blood. Varying from 3-arm 4-arm, 6-arm, 8-arm generated with denser, stiffer structure, increasing elastic modulus 1.5 14.9 kPa, inducing an level immune association (from 15% 45% 8-arm) monocytes. In contrast, precursor template intact (MS@PEG) were generally displayed higher levels but showed opposite trend?immune decreased arm numbers. Proteomics analysis demonstrated biomolecular corona formed minimally influenced interactions, whereas MS@PEG particle–cell correlated composition was abundant histidine-rich glycoproteins. Our work highlights design stealth PEG-based particles, thus providing link between synthetic nature their behavior

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ژورنال

عنوان ژورنال: ACS Nano

سال: 2021

ISSN: ['1936-0851', '1936-086X']

DOI: https://doi.org/10.1021/acsnano.1c01642